2024年2月29日 星期四
根癌农杆菌介导的金边狗牙根遗传转化条件的优化
Optimization of genetic transformation conditions in Cynodon dactylon mediated by Agrobacterium Tumefaciens
2005年 第14卷 第2期 页码[15-18]    下载全文[0.6MB]  
摘要

为建立根癌农杆菌(Agrobacterium tumefaciens,菌株EHA105)介导的金边狗牙根〔Cynodon dactylon(L.)Pers.〕最佳遗传转化体系,以葡萄糖醛酸糖苷酶(GUS)基因的瞬间表达率为指标,从愈伤组织继代时间、根癌农杆菌侵染时间、负压条件及光照时间等方面进行了筛选。结果表明,根癌农杆菌介导的金边狗牙根最佳的转化体系是:以继代培养2周的愈伤组织为起始材料,根癌农杆菌介导感染10min,经负压处理(抽拉20次)并在全光条件下共培养转化。

Abstract

Several factors affecting Agrobacterium tumefaciens EHA 105 mediated genetic transformation of Cynodon dactylon (L.) Pers. were studied based on the variance of GUS (β-glucuronidase) transient expression rate.The results indicated that the optimized conditions are as follows: using the  2-week recultured calli as the original materials, 10 min for infecting with Agrobacterium tumefaciens, negative pressure treatment (pull 20 times by injector), followed by co-culture under continuous light condition.

关键词狗牙根; 遗传转化; 根癌农杆菌; GUS瞬间表达频率;
Key wordsCynodon dactylon (L.) Pers.; genetictrans formation; Agrobacterium tumefaciens; GUS transient expression rate
作者胡繁荣
所在单位浙江省金华职业技术学院生物工程学院,浙江金华321007
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基金项目国家自然科学基金资助项目“彩叶草坪草资源的评价与利用”(30370147);