2024年7月19日 星期五
红籽鸢尾高频离体再生条件的初步筛选
Preliminary selection of high frequency in vitro regeneration condition of Iris foetidissima
2014年 第23卷 第1期 页码[116-118]    下载全文[0.7MB]  
Abstract

Taking stem tips of Iris foetidissima Linn. as explants, effect of different phytohormone combinations on its callus and adventitious bud inductions and effect of different basic media and mass  concentrations of NAA on rooting were studied, the high frequency in vitro regeneration condition of I. foetidissima was selected preliminarily. Results show that the optimum callus induction medium of I. foetidissima is MS medium containing 1. 5 mg·L-1  2,4-D and 0. 1 mg·L-1 6-BA, its callus induction rate is high to 77. 27%. The optimum adventitious bud induction medium is MS medium added with 1. 0 mg·L-1 6-BA and 0. 2 mg·L-1 NAA, with adventitious bud induction rate of 72. 22%. The optimum rooting medium is MS medium added with 0. 2 mg·L-1 NAA, its rooting rate reaches to 100. 00%. The survival rate of plantlets after transplanted is also high with a value of above 95%.

关键词红籽鸢尾; 离体培养; 再生条件
Key wordsIris foetidissima Linn.; in vitro culture; regeneration condition
作者张永侠1, 原海燕2, 顾春笋2, 黄苏珍2
所在单位1. 南京农业大学园艺学院, 江苏南京210095; 2. 江苏省•中国科学院植物研究所(南京中山植物园), 江苏南京210014
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基金项目江苏省科技支撑计划项目(BE2012349)