2024年7月26日 星期五
3 个竹种的花粉特性观察及花粉培养和贮藏条件筛选
Observation on pollen character and selection of pollen culture and storage conditions of three bamboo species
2015年 第24卷 第4期 页码[111-113]    下载全文[1MB]  
Abstract

Pollen character and viability of Shibataea chinensis Nakai, Bambusa multiplex (Lour.) Raeusch. ex Schult. et  J. H. Schult. and Arundinaria simonii f. heterophyllus (Makino et Shirasawa) Muro were observed, and suitable culture and storage conditions of pollen were selected by taking pollen germination rate as the index. Results show that pollen of three bamboo species is nearly spherical with a single aperture, obvious pore ring and cover, aperture is concave. The highest germination rate of S. chinensis, B. multiplex and A. simonii f. heterophyllus is 9. 1%, 15. 8% and 76. 5%, respectively. Suitable culture medium for pollens of S. chinensis and B. multiplex contains boric acid and sucrose with mass ratios of 0. 001% and 15%, respectively, while that for pollen of A. simonii f. heterophyllus contains boric acid and sucrose with mass ratios of 0. 001% and 20%, respectively, and the appropriate culture time is less than 3 h. The suitable storage conditions for pollen of A. simonii f. heterophyllus are storing fresh pollen under 4 ℃or -5℃for less than 10 h.

关键词竹类; 花粉形态; 花粉活力; 花粉萌发率; 培养和贮藏条件
Key wordsbamboo; pollen morphology; pollen viability; pollen germination rate; culture and storage conditions
作者林树燕a,b, 赵荣b, 李洁b, 郑笑b, 丁雨龙a,b,
所在单位南京林业大学: a. 竹类研究所, b. 南方现代林业协同创新中心, 江苏南京210037
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基金项目“十二五”国家科技支撑计划项目(2012BAD23B05); 中国博士后科学基金资助项目(2014M560427); 国家自然科学基金资助项目(31000294); 江苏高校优势学科建设项目(PAPD)