2024年3月29日 星期五
烯效唑对青钱柳试管苗生长及生理特性的影响
Effects of uniconazole on growth and physiological characteristics of Cyclocarya paliurus plantlets
2010年 第19卷 第4期 页码[50-55]    下载全文[0.8MB]  
摘要

在含 0. 00( CK)、0. 01、0. 05、0. 10 和 1. 00 mg· L-1 烯效唑的 WPM 培养基上继代培养 120 d 后,对青钱柳[Cyclocarya paliurus (Batal.) Iljinskaja]试管苗的部分生长及生理指标的变化进行了比较研究。 结果显示:不同质量浓度烯效唑对青钱柳试管苗的生长及生理指标有不同的影响效应。 总体上,随培养基中烯效唑质量浓度的提高,青钱柳试管苗的苗高、叶片数和可溶性蛋白质含量逐渐降低,可溶性糖与可溶性蛋白质含量的比值、SOD 和POD 活性逐渐提高;在培养基中添加 0. 01、0. 05 和 0. 10 mg· L-1烯效唑对青钱柳试管苗的成活率无显著影响,却可使试管苗的单株鲜质量增加量、叶绿素含量和可溶性糖含量均高于对照;在培养基中添加 1. 00 mg· L-1烯效唑能显著或极显著降低试管苗的成活率、单株鲜质量增加量、分化芽数、苗高、叶片数以及叶绿素含量、可溶性糖含量和可溶性蛋白质含量,并使苗茎出现异常增粗和矮化。 而在含 0. 10 mg· L-1烯效唑的培养基上,虽然试管苗的苗高、分化芽数和叶片数分别较对照降低了 28. 03% 、9. 70% 和 12. 37% ,但试管苗的单株鲜质量增加量、叶绿素含量、可溶性糖含量、可溶性糖与可溶性蛋白质含量的比值、 SOD 和 POD 活性分别较对照提高了 99. 39% 、 14. 00% 、5. 00% 、115. 43% 、129. 77% 和33. 79% 。 研究结果表明,在培养基中添加0. 10 mg· L-1烯效唑可有效改善青钱柳试管苗的生长和生理特性,有效控制苗高和叶片数,促进苗茎的增粗,有助于增强试管苗的抗逆能力。

Abstract

Changes of some growth and physiological indexes of Cyclocarya paliurus ( Batal.) Iljinskaja plantlets were comparatively studied after subcultured for 120 d in WPM medium with 0. 00(CK), 0. 01,0. 05, 0. 10 and 1. 00 mg· L-1 uniconazole. The results show that different concentrations of uniconazole have various influences on growth and physiological indexes of C. paliurus plantlets. Generally, seedling height, leaf number and soluble protein content gradually decrease, and ratio of soluble sugar content to soluble protein content, activities of SOD and POD gradually increase with rising of uniconazole concentration in medium. Adding uniconazole of 0. 01, 0. 05 and 0. 10 mg · L-1 in medium has no significant influence on survival rate of plantlets, but leads to higher increment of individual fresh weight and higher contents of chlorophyll and soluble sugar than those in the control. Adding uniconazole of 1. 00 mg· L-1 in medium makes significantly or highly significantly decrease of survival rate, individual fresh weight increment, number of differentiated bud, seedling height, leaf number and contents of chlorophyll, soluble sugar and soluble protein, and also makes abnormal thickening and dwarf of stem of plantlets. While, using the medium with 0. 10 mg· L-1 uniconazole, although seedling height, number of differentiated bud and leaf number of plantlets decrease by 28. 03% , 9. 70% and 12. 37% respectively as compared with those of the control, but individual fresh weight increment, contents of chlorophyll and soluble sugar, ratio of soluble sugar content to soluble protein content, activities of SOD and POD increase by 99. 39% , 14. 00% , 5. 00% , 115. 43% , 129. 77% and 33. 79% respectively as compared with those of the control. It is concluded that adding 0. 10 mg· L-1 uniconazole in medium can effectively improve growth and physiological characteristics of C. paliurus plantlets, control seedling height and leaf number, promote seedling stem thickening, and help to enhance plantlet’s stress resistance.

关键词青钱柳; 试管苗; 烯效唑; 生长; 生理特性
Key wordsCyclocarya paliurus ( Batal.) Iljinskaja; plantlet; uniconazole; growth; physiological characteristics
作者谢寅峰, 王莹, 张志敏, 尚旭岚, 杨万霞, 方升佐
所在单位南京林业大学森林资源与环境学院, 江苏 南京 210037
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基金项目国家林业局林业公益性行业科研专项(200904046); 江苏省高等学校创新团队科研计划资助项目(苏教科[2009]10 号); 江苏省“六大人才高峰”资助项目