2024年7月24日 星期三
四倍体刺槐无性系K 2和K 3的组织培养
Tissue culture of tetraploid clones K 2and K3 of Robinia pseudoacacia
2009年 第18卷 第4期 页码[82-84]    下载全文[0.4MB]  
Abstract

The tissue culture conditions of fast-growing clone K2 and fodder-type clone K3 of Robinia pseudoacacia L. tetraploid were investigated using shoot sprouting in hydroponic growth chamber as explants. After disinfected by 70% ethanol then sterilized with NaClO solution containing 0.5% available chlorine, the explants should be initially cultured with MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar, pH 5.8), and survival rate of K2 and K3 explants all reaches 90% in initial culture medium. The best proliferation medium of K2 adventitious bud is MS medium adding 0.3 mg·L-1 6-BA and 0.05 mg·L-1 NAA, with the highest proliferation coefficient of 5.29,and that of K3 is MS medium adding 1.0 mg·L-1 6-BA and 0.10 mg·L-1 NAA, with the highest proliferation coefficient of 3.36. The optimized rooting medium of K2 and K3 plantlets is 1/2MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar, pH 5.8) adding 0.1 mg·L-1 IBA and 0.1 mg·L-1 NAA, respectively. After training,K2 and K3 plantlets are transplanted by two-step transplanting method, all of survival rate reach 98%. 

关键词刺槐; 四倍体无性系; 组织培养; 不定芽增殖; 生根;
Key wordsRobinia pseudoacacia L.; tetraploid clone; tissue culture; adventitious bud proliferation; rooting
作者张 怡1,2,张俊琦1,罗晓芳1,沈应柏1
所在单位1. 北京林业大学生物科学与技术学院,北京100083;
2.上海应用技术学院科技处,上海200235
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基金项目国家林业局“948”项目(97-4-05);